2024-03-28T19:17:25Zhttp://oai.recercat.cat/request
oai:recercat.cat:2072/392972012-07-10T09:29:54Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorCampàs, Mònica
authorDe la Iglesia, Pablo
authorGiménez Papiol, Gemma
authorFernández-Tejedor, Margarita
authorMarty, Jean-Louis
authorDiogène Fadini, Jorge
2009-07-15T09:22:55Z
2009-07-15T09:22:55Z
2008
2008
Campàs, M. et al. (2008).Marine biotoxins in the Catalan littoral: could biosensors be integrated into monitoring programmes?. Contributions to Science, 4(1):43-53
DOI: 10.2436/20.7010.01.34
http://hdl.handle.net/2072/39297
Aquest article descriu els sensors enzimàtics i immunosensors
electroquímics que s’han desenvolupat als nostres grups per a
la detecció de la biotoxina marina àcid okadaic (OA), i discuteix
la possibilitat d’integrar-los en programes de seguiment. Els
sensors enzimàtics per a OA que es presenten es basen en la
inhibició de la proteïna fosfatasa (PP2A) per aquesta toxina i la
mesura electroquímica de l’activitat enzimàtica mitjançant l’ús
de substrats enzimàtics apropiats, electroquímicament actius
després de la seva desfosforació per l’enzim. Els immunosensors
electroquímics descrits en aquest article es basen en un
enzimoimmunoassaig sobre fase sòlida competitiu indirecte
(ciELISA), amb fosfatasa alcalina (ALP) o peroxidasa (HRP)
com a marcatges, i un sistema de reciclatge enzimàtic amb diaforasa
(DI). Els biosensors presentats aquí s’han aplicat a
l’anàlisi de dinoflagel·lats, musclos i ostres. Les validacions
preliminars amb assaigs colorimètrics i LC-MS/MS han demostrat
la possibilitat d’utilitzar les bioeines desenvolupades
per al cribratge preliminar de biotoxines marines en mostres de
camp o de cultiu, que ofereixen informació complementària a
la cromatografia. En conclusió, tot i que encara cal optimitzar
alguns paràmetres experimentals, la integració dels biosensors
a programes de seguiment és viable i podria proporcionar
avantatges respecte a altres tècniques analítiques pel que fa al
temps d’anàlisi, la simplicitat, la selectivitat, la sensibilitat, el fet
de poder ser d’un sol ús i l’efectivitat de cost.
This article describes the electrochemical enzyme sensors and
immunosensors that have been developed by our groups for
the detection of marine biotoxin okadaic acid (OA), and discusses
the possibility of integrating them into monitoring programmes.
The enzyme sensors for OA reported herein are
based on the inhibition of immobilised protein phosphatase 2A
(PP2A) by this toxin and the electrochemical measurement of
the enzyme activity through the use of appropriate enzyme substrates,
which are electrochemically active after dephosphorylation
by the enzyme. The electrochemical immunosensors described
in this article are based on a competitive indirect Enzyme-
Linked ImmunoSorbent Assay (ciELISA), using alkaline
phosphatase (ALP) or horseradish peroxidase (HRP) as labels,
and an enzymatic recycling system with diaphorase (DI). The
biosensors presented herein have been applied to the analysis
of dinoflagellates, mussels and oysters. Preliminary validations
with colorimetric assays and LC-MS/MS have demonstrated
the possibility of using the developed biotools for the preliminary
screening of marine biotoxins in field or cultured samples, offering
complementary information to chromatography. In conclusion,
although optimisation of some experimental parameters is
still required, the integration of biosensors into monitoring programmes
is viable and may provide advantages over other analytical
techniques in terms of analysis time, simplicity, selectivity,
sensitivity, disposability of electrodes and cost effectiveness.
11 p.
332810 bytes
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eng
Institut d'Estudis Catalans
Aquest document està subjecte a una llicència d'ús de Creative Commons, amb la qual es permet copiar, distribuir i comunicar públicament l'obra sempre que se'n citin l'autor original i l'IRTA i no se'n faci cap ús comercial ni obra derivada, tal com queda estipulat en la llicència d'ús (http://creativecommons.org/licenses/by-nc-nd/2.5/es/)
Algues tòxiques
Marine biotoxins in the Catalan littoral: could biosensors be integrated into monitoring programmes?
info:eu-repo/semantics/article
oai:recercat.cat:2072/394662012-07-10T09:29:54Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorGalimany Sanromà, Eva
authorPlace, Allan R.
authorRamon, Montserrat
authorJutson, Maria
authorPipe, Richard K.
2009-07-17T10:35:34Z
2009-07-17T10:35:34Z
2007-03
2008-01
Galimany, E. et al. (2008). The effects of feeding Karlodinium veneficum (PLY # 103; Gymnodinium veneficum Ballantine) to the blue mussel Mytilus edulis. Harmful Algae 7(1): 91-98
doi:10.1016/j.hal.2007.05.004
http://hdl.handle.net/2072/39466
The effects of exposure to the type species for Karlodinium veneficum (PLY # 103) on immune function and histopathology in the blue mussel Mytilus edulis were investigated. Mussels from Whitsand Bay, Cornwall (UK) were exposed to K. veneficum (PLY # 103) for 3 and 6 days. Assays for immune function included total and differential cells counts, phagocytosis and release of extra cellular reactive oxygen species. Histology was carried out on digestive gland and mantle tissues. The toxin cell quota for K. veneficum (PLY #103) was measured by liquid chromatography-mass spectrometry detecting two separable toxins KvTx1 (11.6 ± 5.4 ng/ml) and KvTx2 (47.7 ± 4.2 ng/ml). There were significant effects of K. veneficum exposure with increasing phagocytosis and release of reactive oxygen species following 6 days exposure. There were no significant effects on total cell counts. However, differential cell counts did show significant effects after 3 days exposure to the toxic alga. All mussels produced faeces but not pseudofaeces indicating that algae were not rejected prior to ingestion. Digestive glands showed ingestion of the algae and hemocyte infiltration after 3 days of exposure, whereas mantle tissue did not show differences between treatments. As the effects of K. veneficum were not observed in the mantle tissue it can be hypothesized that the algal concentration was not high enough, or exposure long enough, to affect all the tissues. Despite being in culture for more than 50 years the original K. veneficum isolate obtained by Mary Parke still showed toxic effects on mussels.
26 p.
189323 bytes
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eng
Elsevier B.V.
Aquest document està subjecte a una llicència d'ús de Creative Commons, amb la qual es permet copiar, distribuir i comunicar públicament l'obra sempre que se'n citin l'autor original i l'IRTA i no se'n faci cap ús comercial ni obra derivada, tal com queda estipulat en la llicència d'ús (http://creativecommons.org/licenses/by-nc-nd/2.5/es/)
Musclos
Algues tòxiques
The effects of feeding Karlodinium veneficum (PLY # 103; Gymnodinium veneficum Ballantine) to the blue mussel Mytilus edulis
info:eu-repo/semantics/article
info:eu-repo/semantics/article
oai:recercat.cat:2072/394682012-07-10T09:29:54Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorGalimany Sanromà, Eva
authorSunila, Inke
authorHégarer, Hélene
authorRamon, Montserrat
authorWikfors, Gary H.
2009-07-17T10:43:30Z
2009-07-17T10:43:30Z
2007
2008-08
Galimany E. (2008). Pathology and immune response of the blue mussel (Mytilus edulis L.) after an exposure to the harmful dinoflagellate Prorocentrum minimum. Harmful Algae 7(5):630-638
doi:10.1016/j.hal.2008.01.001
http://hdl.handle.net/2072/39468
The harmful dinoflagellate Prorocentrum minimum has different effects upon various species of grazing bivalves, and these effects also vary with life-history stage. Possible effects of this dinoflagellate upon mussels have not been reported; therefore, experiments exposing adult blue mussels, Mytilus edulis, to P. minimum were conducted. Mussels were exposed to cultures of toxic P. minimum or benign Rhodomonas sp. in glass aquaria. After a short period of acclimation, samples were collected on day 0 (before the exposure) and after 3, 6, and 9 days of continuous-exposure experiment. Hemolymph was extracted for flow-cytometric analyses of hemocyte, immune-response functions, and soft tissues were excised for histopathology. Mussels responded to P. minimum exposure with diapedesis of hemocytes into the intestine, presumably to isolate P. minimum cells within the gut, thereby minimizing damage to other tissues. This immune response appeared to have been sustained throughout the 9-day exposure period, as circulating hemocytes retained hematological and functional properties. Bacteria proliferated in the intestines of the P. minimum-exposed mussels. Hemocytes within the intestine appeared to be either overwhelmed by the large number of bacteria or fully occupied in the encapsulating response to P. minimum cells; when hemocytes reached the intestine lumina, they underwent apoptosis and bacterial degradation. This experiment demonstrated that M. edulis is affected by ingestion of toxic P. minimum; however, the specific responses observed in the blue mussel differed from those reported for other bivalve species. This finding highlights the need to study effects of HABs on different bivalve species, rather than inferring that results from one species reflect the exposure responses of all bivalves.
33 p.
678662 bytes
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eng
Elsevier B.V.
Aquest document està subjecte a una llicència d'ús de Creative Commons, amb la qual es permet copiar, distribuir i comunicar públicament l'obra sempre que se'n citin l'autor original i l'IRTA i no se'n faci cap ús comercial ni obra derivada, tal com queda estipulat en la llicència d'ús (http://creativecommons.org/licenses/by-nc-nd/2.5/es/)
Musclos
Algues tòxiques
Pathology and immune response of the blue mussel (Mytilus edulis L.) after an exposure to the harmful dinoflagellate Prorocentrum minimum
info:eu-repo/semantics/article
info:eu-repo/semantics/article
oai:recercat.cat:2072/394692012-07-10T09:29:54Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorGalimany Sanromà, Eva
authorSunila, Inke
authorHégaret, Hélène
authorRamón, Montserrat
authorWikfors, Gary H.
2009-07-17T10:49:59Z
2009-07-17T10:49:59Z
2008
2008-08
Galimany, E. et al. (2008). Experimental exposure of the blue mussel (Mytilus edulis, L.) to the toxic dinoflagellate Alexandrium fundyense: Histopathology, immune responses, and recovery. Harmful Algae 7(5):702-711
doi:10.1016/j.hal.2008.02.006
http://hdl.handle.net/2072/39469
Mussels (Mytilus edulis) were exposed to cultures of the toxic dinoflagellate Alexandrium fundyense or the non-toxic alga Rhodomonas sp. to evaluate the effects of the harmful alga on the mussels and to study recovery after discontinuation of the A. fundyense exposure. Mussels were exposed for 9 days to the different algae and then all were fed Rhodomonas sp. for 6 more days. Samples of hemolymph for hemocyte analyses and tissues for histology were collected before the exposure and periodically during exposure and recovery periods.
Mussels filtered and ingested both microalgal cultures, producing fecal pellets containing degraded, partially degraded, and intact cells of both algae. Mussels exposed to A. fundyense had an inflammatory response consisting of degranulation and diapedesis of hemocytes into the alimentary canal and, as the exposure continued, hemocyte migration into the connective tissue between the gonadal follicles. Evidence of lipid peroxidation, similar to the detoxification pathway described for various xenobiotics, was found; insoluble lipofuchsin granules formed (ceroidosis), and hemocytes carried the granules to the alimentary canal, thus eliminating putative dinoflagellate toxins in feces. As the number of circulating hemocytes in A. fundyense-exposed mussels became depleted, mussels were immunocompromised, and pathological changes followed, i.e., increased prevalences of ceroidosis and trematodes after 9 days of exposure. Moreover, the total number of pathological changes increased from the beginning of the exposure until the last day (day 9). After 6 days of the exposure, mussels in one of the three tanks exposed to A. fundyense mass spawned; these mussels showed more severe effects of the toxic algae than non-spawning mussels exposed to A. fundyense.
No significant differences were found between the two treatments during the recovery period, indicating rapid homeostatic processes in tissues and circulating hemocytes.
39 p.
678662 bytes
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eng
Elsevier B.V.
Aquest document està subjecte a una llicència d'ús de Creative Commons, amb la qual es permet copiar, distribuir i comunicar públicament l'obra sempre que se'n citin l'autor original i l'IRTA i no se'n faci cap ús comercial ni obra derivada, tal com queda estipulat en la llicència d'ús (http://creativecommons.org/licenses/by-nc-nd/2.5/es/)
Musclos
Algues tòxiques
Experimental exposure of the blue mussel (Mytilus edulis, L.) to the toxic dinoflagellate Alexandrium fundyense: Histopathology, immune responses, and recovery
info:eu-repo/semantics/article
info:eu-repo/semantics/article
oai:recercat.cat:2072/1726372012-07-10T09:29:54Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorSimeó, Carles G.
authorKurtz, Kathryn
authorChiva, Manel
authorRibes, Enric
authorRotllant Estelrich, Guiomar
2011-11-17T09:08:03Z
2011-11-17T09:08:03Z
2010
2010
Simeó, C.G., et al.(2010). "Spermatogenesis of the Spider Crab Maja brachydactyla (Decapoda: Brachyura)" Journal of morphology, 271 (4) p.394-406
http://hdl.handle.net/2072/172637
This study describes spermatogenesis in a
majid crab (Maja brachydactyla) using electron microscopy
and reports the origin of the different organelles
present in the spermatozoa. Spermatogenesis in M. brachydactyla
follows the general pattern observed in other
brachyuran species but with several peculiarities. Annulate
lamellae have been reported in brachyuran spermatogenesis
during the diplotene stage of first spermatocytes,
the early and mid-spermatids. Unlike previous
observations, a Golgi complex has been found in midspermatids
and is involved in the development of the
acrosome. The Golgi complex produces two types of
vesicles: light vesicles and electron-dense vesicles. The
light vesicles merge into the cytoplasm, giving rise to
the proacrosomal vesicle. The electron-dense vesicles are
implicated in the formation of an electron-dense granule,
which later merges with the proacrosomal vesicle. In the
late spermatid, the endoplasmic reticulum and the Golgi
complex degenerate and form the structures–organelles
complex found in the spermatozoa. At the end of spermatogenesis,
the materials in the proacrosomal vesicle
aggregate in a two-step process, forming the characteristic
concentric three-layered structure of the spermatozoon
acrosome. The newly formed spermatozoa from testis
show the typical brachyuran morphology.
40
3539821 bytes
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Aquest document està subjecte a una llicència d'ús de Creative Commons, amb la qual es permet copiar, distribuir i comunicar públicament l'obra sempre que se'n citin l'autor original i l'IRTA i no se'n faci cap ús comercial ni obra derivada, tal com queda estipulat en la llicència d'ús (http://creativecommons.org/licenses/by-nc-nd/2.5/es/)
Crustacis -- Reproducció
Espermatogènesi
Spermatogenesis of the Spider Crab Maja brachydactyla (Decapoda: Brachyura)
info:eu-repo/semantics/article
oai:recercat.cat:2072/1726382012-07-10T09:29:54Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorSimeó, Carles G.
authorKurtz, Kathryn
authorRotllant Estelrich, Guiomar
authorChiva, Manel
authorRibes, Enric
2011-11-17T09:51:43Z
2011-11-17T09:51:43Z
2010
2010
Simeó, Carles G., et al. (2010). " Sperm Ultrastructure of the Spider Crab Maja brachydactyla (Decapoda: Brachyura)" Journal of morphology, 271 (4) p. 407-417
http://hdl.handle.net/2072/172638
This study describes the morphology of the
sperm cell of Maja brachydactyla, with emphasis on localizing
actin and tubulin. The spermatozoon of M. brachydactyla
is similar in appearance and organization to other
brachyuran spermatozoa. The spermatozoon is a globular
cell composed of a central acrosome, which is surrounded
by a thin layer of cytoplasm and a cup-shaped nucleus
with four radiating lateral arms. The acrosome is a subspheroidal
vesicle composed of three concentric zones surrounded
by a capsule. The acrosome is apically covered by
an operculum. The perforatorium penetrates the center of
the acrosome and has granular material partially composed
of actin. The cytoplasm contains one centriole in
the subacrosomal region. A cytoplasmic ring encircles the
acrosome in the subapical region of the cell and contains
the structures-organelles complex (SO-complex), which is
composed of a membrane system, mitochondria with few
cristae, and microtubules. In the nucleus, slightly condensed
chromatin extends along the lateral arms, in
which no microtubules have been observed. Chromatin
fibers aggregate in certain areas and are often associated
with the SO-complex. During the acrosomal reaction, the
acrosome could provide support for the penetration of the
sperm nucleus, the SO-complex could serve as an anchor
point for chromatin, and the lateral arms could play an
important role triggering the acrosomal reaction, while
slightly decondensed chromatin may be necessary for the
deformation of the nucleus.
31
1571266 bytes
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Aquest document està subjecte a una llicència d'ús de Creative Commons, amb la qual es permet copiar, distribuir i comunicar públicament l'obra sempre que se'n citin l'autor original i l'IRTA i no se'n faci cap ús comercial ni obra derivada, tal com queda estipulat en la llicència d'ús (http://creativecommons.org/licenses/by-nc-nd/2.5/es/)
Espermatogènesi -- Aspectes genètics
Embriologia -- Crustacis
Sperm Ultrastructure of the Spider Crab Maja brachydactyla (Decapoda: Brachyura)
info:eu-repo/semantics/article
oai:recercat.cat:2072/1727132012-07-10T09:29:54Zhdl_2072_13341
oai:recercat.cat:2072/1729102011-11-30T09:32:49Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorAndrés Roig, Mireia
authorGisbert Casas, Enric
authorDíaz, Manuel
authorMoyano, Francisco J.
authorEstévez García, Alicia
authorRotllant Estelrich, Guiomar
author
2011-11-29T10:36:30Z
2011-11-29T10:36:30Z
2010
2010
Andres, M. et al (2010) " Ontogenetic changes in digestive enzymatic capacities of the spider crab, Maja brachydactyla (Decapoda: Majidae)" Journal of experimental Marine Biology and Ecology, 389 (1-2) p.75-84
http://hdl.handle.net/2072/172910
Ontogenetic changes in digestive capabilities were analyzed in larvae and first juveniles of the spider crab Maja brachydactyla. Activities of five proteinases (total proteases, trypsin, chymotrypsin, pepsin-like and aminopeptidase), three carbohydrases (amylase, maltase and chitinase), an esterase and an alkaline phosphatase were studied to evaluate digestive enzyme profiles of the species. Both quantitative (spectrophotometry and fluorometry) and qualitative (SDS-PAGE) approaches were used. All assayed enzymes were active from hatching (zoea I-ZI) throughout larval development and in first juveniles. Significant variations during ontogeny were found only in total activities likely as a consequence of digestive system development. Specific activity varied little over ontogeny, being significant only for chitinase. Total proteases, trypsin and pepsin-like activities showed a similar pattern of increase as larval ontogeny advanced, decreasing significantly in juveniles. Chymotrypsin continued to increase, showing maximum activity after metamorphosis. Proteinase zymograms confirmed strong proteolytic activity in first zoeas, with increasing bands over the course of ontogeny, decreasing after metamorphosis. A group of bands with high molecular mass was specific to larval stages. Amylase and maltase showed a parallel pattern of continuous increase of total activity as development advanced. Gel-SDS-PAGE showed unchanged patterns of amylase activity in first zoeas of different ages and the most complex set of bands during larval ontogeny in second zoea. Esterase total activity increased significantly as ZI's aged likely reflecting introduction of a lipid-enriched diet. The importance of lipid accumulation at the beginning of ontogeny was also confirmed by the protease/esterase and amylase/esterase activity ratios, which decreased from hatch to late ZI and might be explained as an adaptation, ensuring the next molt. The results suggest that larvae of M. brachydactyla are capable of digesting a variety of dietary substrates as soon as they hatch.
45 p.
eng
Reproducció del document publicat a : http://dx.doi.org/doi:10.1016/j.jembe.2010.03.015
info:eu-repo/semantics/openAccess
L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/
RECERCAT (Dipòsit de la Recerca de Catalunya)
Crustacis -- Cria i desenvolupament
Aqüicultura
Ontogenetic changes in digestive enzymatic capacities of the spider crab, Maja brachydactyla (Decapoda: Majidae)
info:eu-repo/semantics/article
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtc2EvMy4wL2VzLw==
oai:recercat.cat:2072/1729092011-11-30T09:34:12Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorAndrés Roig, Mireia
authorRotllant Estelrich, Guiomar
authorZeng, Chaoshu
2011-11-29T10:36:29Z
2011-11-29T10:36:29Z
2010
2010-02
Andrés, M. (et al.) (2010) Survival, development and growth of larvae of the blue swimmer crab, Portunus pelagicus, cultured under different photoperiod conditions. Aquaculture 300 (1-4) : 218-222
http://hdl.handle.net/2072/172909
The blue swimmer crab is a commercially important species of the tropical Indo-Pacific regions that shows substantial potential as a candidate species for aquaculture. Optimization of larval rearing conditions, including photoperiod, is therefore important to establish a method for the intensive hatchery culture of this species. Newly hatched larvae of Portunuspelagicus in first zoeal stage (ZI) were reared under five photoperiod regimes 0L: 24D, 6L: 18D, 12L: 12D, 18L: 6D, and 24L: 0D (5 replicates per treatment) till they metamorphosed to megalopae (ranged from 8.5 ± 0.3 days (18L: 6D) to 10.8 ± 1.8 days (0L: 24D) at 29 ± 1 °C). Daily, larvae of each treatment were fed an identical diet of mixed rotifer and Artemia nauplii, and the survival and molt to successive stages was monitored.
Newly hatched ZI larvae of P. pelagicus could successfully develop to the megalopal stage under all tested photoperiod conditions, but we detected significant differences in survival among treatments (p & 0.05). The constant darkness treatment (0L: 24D) had the lowest (19.2 ± 7.2%, mean ± S.E.) cumulative survival from ZI to the megalopal stage, while the 18L: 6D treatment achieved the highest survival (51.2 ± 23.6%). Similarly, the photoperiod significantly affected zoeal development. Constant darkness led to the longest cumulative zoeal duration (10.8 ± 1.8 days), whereas the 18L: 6D treatment rendered the shortest larval development (8.5 ± 0.3 days). In addition, larvae reared under constant darkness resulted in the smallest megalopae (carapace length = 1.44 ± 0.09 mm) and the lowest dry weight (0.536 ± 0.188 mg).
In conclusion, photoperiod significantly affected the survival, development, and growth of P. pelagicus zoeal larvae. Constant darkness led to the lowest larval survival and developmental rate, while a photoperiod regime of 18L: 6D appeared to be the most suitable condition for the rearing of zoeal larvae of P. pelagicus.
19 p.
eng
Elsevier
Reproducció del document publicat a: http://dx.doi.org/10.1016/j.aquaculture.2009.12.026
info:eu-repo/semantics/openAccess
L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/
RECERCAT (Dipòsit de la Recerca de Catalunya)
Aqüicultura
Crustacis -- Cria i desenvolupament
Survival, development and growth of larvae of the blue swimmer crab, Portunus pelagicus, cultured under different photoperiod conditions
info:eu-repo/semantics/article
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtc2EvMy4wL2VzLw==
oai:recercat.cat:2072/1729152011-12-07T12:42:17Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorRamirez-Llodra, Eva
authorCompany, Joan B.
authorCamps, Mercedes
authorRotllant Estelrich, Guiomar
2011-11-29T12:02:13Z
2011-11-29T12:02:13Z
2007
2007
Ramirez-Llodra, E. et al (2007). "Spatio-temporal variations in reproductive patterns and population structure of Pasiphaea multidentata (Decapoda: Caridea) in the Blanes canyon and adjacent margin, North-western Mediterranean Sea" Marine Ecology, 28 (4) p.470-479
http://hdl.handle.net/2072/172915
The bathyal faunal communities of the NW Mediterranean slopes have been studied consistently in the last two decades, with a special focus on population structure, trophic dynamics and benthopelagic coupling of commercial deep-sea decapod crustaceans and fishes (reviewed in Sardà et al. 2004) and associated species (Cartes and Sardà, 1993; Company and Sardà, 1997, 2000; Cartes et al., 2001; Company et al., 2001, 2003, 2004). One of the major topographic features in the North-western Mediterranean slope is the presence of submarine canyons. Canyons play a major role in funnelling energy and organic matter from the shelf to bathyal and abyssal depths (Puig et al., 2000), but the implications of this enhanced organic supply in the deep-sea benthic communities is still mostly unknown. Trophic supply can follow two major pathways – vertical deposition in the water column (Billett et al., 1983; Baldwin et al., 1998; Lampitt et al., 2001) or down-slope advection on the margins (Puig et al., 2001; Bethoux et al., 2002; Canals et al., 2006) – and can be a limiting factor in the deep-sea, being especially important in the oligotrophic Mediterranean Sea (Sardà et al., 2004). Differences in the quantity, quality and timing of organic matter input to the deep seafloor have been used to explain patterns of biomass and abundance in benthic communities (Levin et al., 1994; Gooday & Turley, 1990; Billett et al., 2001; Galéron et al., 2001; Puig et al., 2001; Gage, 2003) as well as other biological process and in particular the existence of seasonal reproduction (Tyler et al., 1994; Company et al., 2004 (MEPS). Reproduction is a highly energetic process tightly linked to food availability and quality.
20 p.
eng
Wiley-Blackwell
Reproducció del document publicat a: http://dx.doi.org/10.1111/j.1439-0485.2007.00207.x
info:eu-repo/semantics/openAccess
L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/
RECERCAT (Dipòsit de la Recerca de Catalunya)
Aqüicultura -- Catalunya
Crustacis -- Catalunya
Spatio-temporal variations in reproductive patterns and population structure of Pasiphaea multidentata (Decapoda: Caridea) in the Blanes canyon and adjacent margin, North-western Mediterranean Sea
info:eu-repo/semantics/article
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtc2EvMy4wL2VzLw==
oai:recercat.cat:2072/1729412012-07-04T11:02:57Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorGuerao Serra, Guillermo
authorRotllant Estelrich, Guiomar
2011-11-30T14:41:23Z
2011-11-30T14:41:23Z
2010
2010
Guerao, G., Rotllant, G. (2010) " Development and growth of the early juveniles of the spider crab Maja squinado (Brachyura: Majoidea) in an individual culture system" Aquaculture 307 (1-2) p. 105-110
http://hdl.handle.net/2072/172941
The spider crab Maja squinado is an endangered Mediterranean species; therefore, culturing it successfully is essential for developing restocking programs. The survival, growth and development of post-larval stages (juvenile crabs, C1-C8) were studied using larvae obtained from adult individuals collected in the Catalan Sea. The juvenile crab stages were cultured individually from a megalopal stage using a semi-open recirculation system to obtain the precise growth data of each juvenile crab stage until C8. Development up to C8 at 20ºC lasted 154±10 days. Survival from C1 to C8 was 5.8 %. Moult increment values in cephothoracic length were similar in all the crab stages (21-35 %). Intermoult duration (9±1 in C1-C2 to 51±8 days in C7-C8) increased sharply from juvenile stage 5. Males and females can be distinguished from C4 based on sexual dimorphism in the pleopods and the presence of gonopores. The allometric growth of the pleon is sex-dependent from C4, with females showing positive allometry and males isometric growth. The juvenile growth rate was lower compared with that of the previously studied Atlantic species Maja brachydactyla.
28 p.
eng
Elsevier
Reproducció del document publicat a:http://dx.doi.org/10.1016/j.aquaculture.2010.06.006
info:eu-repo/semantics/openAccess
L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/
RECERCAT (Dipòsit de la Recerca de Catalunya)
Crustacis -- Catalunya
Development and growth of the early juveniles of the spider crab Maja squinado (Brachyura: Majoidea) in an individual culture system
info:eu-repo/semantics/article
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtc2EvMy4wL2VzLw==
oai:recercat.cat:2072/1729442012-07-04T10:44:59Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorGomez-Gil, Bruno
authorRoque, A.
authorLacuesta, B.
authorRotllant Estelrich, Guiomar
2011-11-30T15:25:08Z
2011-11-30T15:25:08Z
2010
2010
Gomez-Gil, B. et al. (2010) " Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla." Journal of Applied Microbiology, 109 (3) p. 918-926
http://hdl.handle.net/2072/172944
Aims: The aim of this study was to characterise and identify vibrios isolated
from the haemolymph of apparently healthy adult spider crabs (Maja brachydactyla)
wild-caught in the Spanish localities of Galician coast and in the Canary
Islands and also from captive animals held at IRTA’s facilities in the Ebro
Delta of Catalonia, north-west Spanish Mediterranean coast.
Methods and Results: A total of 277 bacterial isolates were obtained, and of
these, 171 were characterised with rep-PCR, resulting electrophoretic bands
were analysed and clusters formed. Identification of representative strains of
each cluster was made by sequencing the 16S rRNA. Samples from animals
caught in Galicia and captive at IRTA (around 15–18 C) rendered mostly
species belonging to the Splendidus clade (72Æ2 and 76Æ6% respectively),
commonly found in cold waters (below 20 C). Higher species diversity was
found in the haemolymph of the captive animals. In the warmer Canary
Islands waters (around 21 C), the diversity of vibrios is dominated by three
clades, Harveyi (Vibrio core group, 39Æ3%), Orientalis (23Æ2%) and Splendidus
(21Æ4%) with a species diversity that equals that of the colder captive animals.
Conclusions: Differences in the vibrios populations were found in the
haemolymph extracted from animals collected from the three localities. Potential
new species were found, and their description is under way.
Significance and Impact of Study: As with other invertebrates, spider crabs also
contain a diverse population of vibrios. These findings should help researchers
to diagnose when a crab is infected.
28 p.
eng
Wiley-Blackwell
Reproducció del document publicat a:http://dx.doi.org/10.1111/j.1365-2672.2010.04718.x
info:eu-repo/semantics/openAccess
L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/
RECERCAT (Dipòsit de la Recerca de Catalunya)
Crustacis -- Conservació
Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla
info:eu-repo/semantics/article
oai:recercat.cat:2072/1729602012-07-04T11:00:23Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorGuerao Serra, Guillermo
authorRotllant Estelrich, Guiomar
authorAnger, Klaus
2011-12-01T13:55:37Z
2011-12-01T13:55:37Z
2009
2010
Guerao, G. et al. (2010) "Characterization of larval moulting cycles in Maja brachydactyla (Brachyura, Majidae) reared in the laboratory" Aquaculture 302 (1-2) p.106-111
http://hdl.handle.net/2072/172960
The moulting cycles of all larval instars (zoea I, zoea II, and megalopa) of the spider crab Maja brachydactyla
Balss 1922 were studied in laboratory rearing experiments. Morphological changes in the epidermis and
cuticle were photographically documented in daily intervals and assigned to successive stages of the
moulting cycle (based on Drach's classification system). Our moult-stage characterizations are based on
microscopical examination of integumental modifications mainly in the telson, using epidermal condensation,
the degree of epidermal retraction (apolysis), and morphogenesis (mainly setagenesis) as criteria. In
the zoea II and megalopa, the formation of new setae was also observed in larval appendages including the
antenna, maxillule, maxilla, second maxilliped, pleopods, and uropods. As principal stages within the zoea I
moulting cycle, we describe postmoult (Drach's stages A–B combined), intermoult (C), and premoult (D), the
latter with three substages (D0, D1, and D2). In the zoea II and megalopa, D0 and D1 had to be combined,
because morphogenesis (the main characteristic of D1) was unclear in the telson and did not occur
synchronically in different appendices. The knowledge of the course and time scale of successive moult-cycle
events can be used as a tool for the evaluation of the developmental state within individual larval instars,
providing a morphological reference system for physiological and biochemical studies related to crab
aquaculture.
20 p.
eng
Elsevier
Reproducció del document publicat a : http://dx.doi.org/10.1016/j.aquaculture.2010.02.010
info:eu-repo/semantics/openAccess
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RECERCAT (Dipòsit de la Recerca de Catalunya)
Crustacis -- Cria i desenvolupament
Characterization of larval moulting cycles in Maja brachydactyla (Brachyura, Majidae) reared in the laboratory
info:eu-repo/semantics/article
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtc2EvMy4wL2VzLw==
oai:recercat.cat:2072/1790842012-07-04T10:52:28Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorFernández, Margarita
authorDelgado, Maximino
authorGarcés, E.
authorCamp, Jordi
authorDiogène Fadini, Jorge
2011-12-13T12:56:47Z
2011-12-13T12:56:47Z
2010
2010
Fernández-Tejedor, Margarita, Maximino Delgado, Esther Garcés, Jordi Camp, Jorge Diogène (2010) Toxic phytoplankton response to warming in two Mediterranean bays of the Ebro Delta. In: CIESM, 2010 Phytoplankton responses to Mediterranean environmental changes Nº 40 CIESM Workshop Monographs (Ed. F Briand) 83-88
http://hdl.handle.net/2072/179084
The analysis of the phytoplankton and environmental parameters of the time series in Alfacs and Fangar bays (north western Mediterranean) from 1990 to 2009 shows some trends. There is an increase in the average water column temperature, 0.11, 0.01, 0.80 and 0.23 ºC for spring, summer, fall and winter respectively in Alfacs Bay and 1.76, 0.71, 1.33, 0.89 ºC for spring, summer, fall and winter in Fangar Bay. The trends in phytoplankton populations show a shift in the timing of occurrence of Karlodinium spp. blooms and an increase of the Pseudo-nitzschia spp. abundances. There is a lack of correlation between the average seasonal temperatures and the toxic phytoplankton abundances.
33 p.
eng
info:eu-repo/semantics/openAccess
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RECERCAT (Dipòsit de la Recerca de Catalunya)
Fitoplàncton -- Mediterrània
Toxic phytoplankton response to warming in two Mediterranean bays of the Ebro Delta
info:eu-repo/semantics/article
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtc2EvMy4wL2VzLw==
oai:recercat.cat:2072/1790852012-07-04T10:47:20Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorEstévez García, Alicia
authorSimeó, Carles G.
authorRotllant Estelrich, Guiomar
author Andrés Roig, Mireia
2011-12-13T15:11:46Z
2011-12-13T15:11:46Z
2010
2010-12
Andrés M. et al (2010) " Annual variation in the biochemical composition of newly hatched larvae of Maja brachydactyla in captivity" Aquaculture, 310 (1-2) p.99-105
http://hdl.handle.net/2072/179085
Quality of newly hatched larvae (NHL) of Maja brachydactyla in captivity has been characterized throughout the year to evaluate their availability for mass production. Spawning took place every month and NHL were collected and analyzed to estimate individual dry weight (DW) and proximate biochemical composition (protein, carbohydrate and lipids). Lipid class, fatty acid composition, amino acid profile, mineral and vitamins A, E and C contents were analyzed seasonally. NHL obtained throughout the year are a potential source for aquaculture purposes, since the increment in the relative protein and lipid (especially phospholipids and n-3 PUFA) content might compensate the decrease in DW of larvae hatched from broodstock kept during one year in captivity. However, the decrease in vitamins A and E as well as in certain essential amino acids (Lys, Val, and His) and trace elements (Cu and Fe) of NHL at the end of the year might be indicative of a nutritional deficiency in broodstock diets.
31 p.
eng
Elsevier
Reproducció del document publicat a: http://dx.doi.org/10.1016/j.aquaculture.2010.09.035
info:eu-repo/semantics/openAccess
L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/
RECERCAT (Dipòsit de la Recerca de Catalunya)
Crustacis
Annual variation in the biochemical composition of newly hatched larvae of Maja brachydactyla in captivity
info:eu-repo/semantics/article
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtc2EvMy4wL2VzLw==
oai:recercat.cat:2072/1792392012-07-04T10:46:45Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorAlves Martins, Dulce
authorRocha, Filipa
authorMartínez Rodríguez, Gonzalo
authorBell, Gordon
authorJacinto Morais, Sofia
authorCastanheira, Filipa
authorBandarra, Narcisa
authorCoutinho, Joana
authorYúfera, Manuel
authorConceição, Luís E. C.
2012-01-04T14:01:28Z
2012-01-04T14:01:28Z
2011
2011-12
Alves, D. et al. "Teleost fish larvae adapt to dietary arachidonic acid supply through modulation of the expression of lipid metabolism and stress response genes" British Journal of Nutrition 15(2011) p. 1-11
http://hdl.handle.net/2072/179239
Dietary fatty acid supply can affect stress response in fish during early development. Although knowledge on the mechanisms involved in fatty acid regulation of stress tolerance is scarce, it has often been hypothesised that eicosanoid profiles can influence cortisol production. Genomic cortisol actions are mediated by cytosolic receptors which may respond to cellular fatty acid signalling. An experiment was designed to test the effects of feeding gilthead sea-bream larvae with four microdiets, containing graded arachidonic acid (ARA) levels (0·4, 0·8, 1·5 and 3·0 %), on the expression of genes involved in stress response (steroidogenic acute regulatory protein, glucocorticoid receptor and phosphoenolpyruvate carboxykinase), lipid and, particularly, eicosanoid metabolism (hormone-sensitive lipase, PPARα, phospholipase A2, cyclo-oxygenase-2 and 5-lipoxygenase), as determined by real-time quantitative PCR. Fish fatty acid phenotypes reflected dietary fatty acid profiles. Growth performance, survival after acute stress and similar whole-body basal cortisol levels suggested that sea-bream larvae could tolerate a wide range of dietary ARA levels. Transcription of all genes analysed was significantly reduced at dietary ARA levels above 0·4 %. Nonetheless, despite practical suppression of phospholipase A2 transcription, higher leukotriene B4 levels were detected in larvae fed 3·0 % ARA, whereas a similar trend was observed regarding PGE2 production. The present study demonstrates that adaptation to a wide range of dietary ARA levels in gilthead sea-bream larvae involves the modulation of the expression of genes related to eicosanoid synthesis, lipid metabolism and stress response. The roles of ARA, other polyunsaturates and eicosanoids as signals in this process are discussed.
41 p.
eng
Cambridge University Press
Reproducció del document publicat a: http://dx.doi.org/10.1017/S0007114511006143
info:eu-repo/semantics/openAccess
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RECERCAT (Dipòsit de la Recerca de Catalunya)
Peixos
Teleost fish larvae adapt to dietary arachidonic acid supply through modulation of the expression of lipid metabolism and stress response genes
info:eu-repo/semantics/article
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtc2EvMy4wL2VzLw==
oai:recercat.cat:2072/1963002012-07-04T11:02:13Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorBen Naila, Idriss
authorHamza, Asma
authorGdoura, Radhouane
authorDiogène Fadini, Jorge
authorde la Iglesia, Pablo
2012-05-30T11:09:50Z
2012-05-30T11:09:50Z
2011
2012
Ben Naila, I., et al. (2012) Prevalence and persistence of gymnodimines in clams from the Gulf of Gabes (Tunisia) studied by mouse bioassay and LC–MS/MS. Harmful Algae, 18:56-64. http://dx.doi.org/10.1016/j.hal.2012.04.004
http://hdl.handle.net/2072/196300
In this work we studied the toxicity in clams from the Gulf of Gabes, Tunisia (Southern
Mediterranean). Samples from two stations (M2 and S6) were collected monthly from
January 2009 to September 2010, and analyzed by the official control method of
mousse bioassay (MBA) for lipophilic toxins. All samples were also analyzed with the
LC-MS/MS method for the determination of lipophilic toxins, namely: okadaic acid
group, pectenotoxins, yessotoxins and azaspiracids, spirolides and gymnodimines
(GYMs). The results showed prevalence of GYMs since it was the only toxin group
identified in these samples with a maximum of 2,136 μg GYM -A kg-1 (February 2009
at M2). Furthermore, GYMs showed persistence in the area, with only one blank sample
below the limit of detection. Interestingly, this blank sample was found in June 2009
after an important toxic episode which supports the recent findings regarding the high
detoxification capability of clams, much faster than that reported for oysters. In
comparison, good agreement was found among MBA, the LD50 value of 80-100 μg kg-1
reported for GYM- A, and quantitative results provided by LC-MS/MS. On the contrary
to that previously reported for Tunisian clams, we unambiguously identified and
quantified by LC-MS/MS the isomers GYM- B/C in most samples. Phytoplankton
identification and enumeration of Karenia selliformis usually showed higher densities at
site M2 than S6 as expected bearing in mind toxin results, although additional results
would be required to improve the correlation between K. selliformis densities and
quantitative results of toxins. The prevalence and persistence of GYMs in this area at
high levels strongly encourages the evaluation of the chronic toxic effects of GYMs.
This is especially important taking into account that relatively large quantities of GYMs
can be released into the market due to the replacement of the official control method
from mouse bioassay to the LC-MS/MS for lipophilic toxins (Regulation (EU) No
15/2011), and the lack of Regulation for this group of toxins.
22 p.
eng
Elsevier
info:eu-repo/semantics/openAccess
L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/
RECERCAT (Dipòsit de la Recerca de Catalunya)
Toxines marines -- Tunísia
Prevalence and persistence of gymnodimines in clams from the Gulf of Gabes (Tunisia) studied by mouse bioassay and LC-MS/MS
info:eu-repo/semantics/article
info:eu-repo/semantics/article
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtc2EvMy4wL2VzLw==
oai:recercat.cat:2072/2064542017-07-19T07:33:05Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorGarcía-Altares, María
authorDiogène Fadini, Jorge
authorDe la Iglesia González, Pablo de la
2013-02-07T15:38:03Z
2013-02-07T15:38:03Z
2012
2013
García-Altares, M., et al. (2013) The implementation of liquid chromatography tandem mass spectrometry for the official control of lipophilic toxins in seafood: Single-laboratory validation under four chromatographic conditions. Journal of Chromatrography A, 1275: 48-60
http://hdl.handle.net/2072/206454
We performed a comprehensive study to assess the fit for purpose of four chromatographic conditions for the determination of six groups of marine lipophilic toxins (okadaic acid and dinophysistoxins, pectenotoxins, azaspiracids, yessotoxins, gymnodimine and spirolides) by LC-MS/MS to select the most suitable conditions as stated by the European Union Reference Laboratory for Marine Biotoxins (EURLMB). For every case, the elution gradient has been optimized to achieve a total run-time cycle of 12 min. We performed a single-laboratory validation for the analysis of three relevant matrices for the seafood aquaculture industry (mussels, pacific oysters and clams), and for sea urchins for which no data about lipophilic toxins have been reported before. Moreover, we have compared the method performance under alkaline conditions using two quantification strategies: the external standard calibration (EXS) and the matrix-matched standard calibration (MMS). Alkaline conditions were the only scenario that allowed detection windows with polarity switching in a 3200 QTrap mass spectrometer, thus the analysis of all toxins can be accomplished in a single run, increasing sample throughput. The limits of quantification under alkaline conditions met the validation requirements established by the EURLMB for all toxins and matrices, while the remaining conditions failed in some cases. The accuracy of the method and the matrix effects where generally dependent on the mobile phases and the seafood species. The MMS had a moderate positive impact on method accuracy for crude extracts, but it showed poor trueness for seafood species other than mussels when analyzing hydrolyzed extracts. Alkaline conditions with EXS and recovery correction for OA were selected as the most proper conditions in the context of our laboratory. This comparative study can help other laboratories to choose the best conditions for the implementation of LC-MS/MS according to their own necessities.
29 p.
eng
Elsevier
info:eu-repo/semantics/openAccess
L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-nd/3.0/es/
RECERCAT (Dipòsit de la Recerca de Catalunya)
Toxines marines
Espectrometria de masses
Cromatografia líquida
The implementation of liquid chromatography tandem mass spectrometry for the official control of lipophilic toxins in seafood: Single-laboratory validation under four chromatographic conditions
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtbmQvMy4wL2VzLw==
oai:recercat.cat:2072/2072142017-07-19T08:39:47Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorGaribo, Diana
authorDàmaso, Esther
authorEixarch Puigcerver, Helena
authorDe la Iglesia González, Pablo de la
authorFernández-Tejedor, Margarita
authorDiogène Fadini, Jorge
authorPazos, Yolanda
authorCampàs i Homs, Mònica
2013-02-22T12:31:42Z
2013-02-22T12:31:42Z
2012
2013-02-22
Garibo, D., et al. (2012). Protein phosphatase inhibition assays for okadaic acid detection in shellfish: matrix effects, applicability and comparison with LC-MS/MS analysis. Harmful Algae 19:68-75 http://dx.doi.org/10.1016/j.hal.2012.06.001
http://hdl.handle.net/2072/207214
The applicability of the protein phosphatase inhibition assay (PPIA) to the determination of okadaic acid
(OA) and its acyl derivatives in shellfish samples has been investigated, using a recombinant PP2A and a
commercial one. Mediterranean mussel, wedge clam, Pacific oyster and flat oyster have been chosen as
model species. Shellfish matrix loading limits for the PPIA have been established, according to the
shellfish species and the enzyme source. A synergistic inhibitory effect has been observed in the presence
of OA and shellfish matrix, which has been overcome by the application of a correction factor (0.48).
Finally, Mediterranean mussel samples obtained from Rı´a de Arousa during a DSP closure associated to
Dinophysis acuminata, determined as positive by the mouse bioassay, have been analysed with the PPIAs.
The OA equivalent contents provided by the PPIAs correlate satisfactorily with those obtained by liquid
chromatography–tandem mass spectrometry (LC–MS/MS).
35 p.
eng
Elsevier
info:eu-repo/semantics/openAccess
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RECERCAT (Dipòsit de la Recerca de Catalunya)
Toxines marines
Espectrometria de Mases
Cromatografia Líquida
Protein phosphatase inhibition assays for okadaic acid detection in shellfish: matrix effects, applicability and comparison with LC-MS/MS analysis
info:eu-repo/semantics/article
info:eu-repo/semantics/draft
aHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMtbmQvMy4wL2VzLw==
oai:recercat.cat:2072/2101872017-07-19T08:42:47Zhdl_2072_13341
RECERCAT (Diposit de la Recerca de Catalunya)
IRTA. Recerca i Tecnologia Agroalimentàries
authorGaribo, Diana
authorDevic, Eric
authorMarty, Jean-Louis
authorDiogène Fadini, Jorge
authorUnzueta, Idoia
authorBlázquez, María
authorCampàs i Homs, Mònica
2013-04-19T08:02:20Z
2013-04-19T08:02:20Z
2011-12
2012-01
Garibo, D., et al. (2012). Conjugation of genetically-engineered protein phosphatases to magnetic particles for okadaic acid detection. Journal of Biotechnology 157(1):89-95 doi: 10.1016/j.jbiotec.2011.11.020.
http://hdl.handle.net/2072/210187
This work presents the functional characterisation of a protein phosphatase 2A (PP2A) catalytic subunit obtained by genetic engineering and its conjugation to magnetic particles (MPs) via metal coordination chemistry for the subsequent development of assays for diarrheic lipophilic marine toxins. Colorimetric assays with free enzyme have allowed the determination of the best enzyme activity stabiliser, which is glycerol at 10%. They have also demonstrated that the recombinant enzyme can be as sensitive towards okadaic acid (OA) (LOD=2.3μg/L) and dinophysistoxin-1 (DTX-1) (LOD=15.2μg/L) as a commercial PP2A and, moreover, it has a higher operational stability, which makes possible to perform the protein phosphatase inhibition assay (PPIA) with a lower enzyme amount. Once conjugated to MPs, the PP2A catalytic subunit still retains its enzyme activity and it can also be inhibited by OA (LOD=30.1μg/L).
29 p.
eng
Journal of Biotechnology
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Toxines marines
Conjugation of genetically-engineered protein phosphatases to magnetic particles for okadaic acid detection
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